Favouring the bioavailability of Zn and Cu to enhance the production of lignin-modifying enzymes in Trametes versicolor cultures - INRA - Institut national de la recherche agronomique Accéder directement au contenu
Article Dans Une Revue Bioresource Technology Année : 2011

Favouring the bioavailability of Zn and Cu to enhance the production of lignin-modifying enzymes in Trametes versicolor cultures

Résumé

Metal effect on the enzyme secretion in fungi is usually related to total concentrations but not to bioavailable metal species. In this work, we aimed at enhancing the secretion of lignin-modifying oxidoreductases in Trametes versicolor by favouring the bioavailability of essential metals. For this purpose, the fungus was exposed to Cu or Zn in liquid culture media exhibiting different complexation levels. Metal speciation was determined experimentally or theoretically to quantify free metal species, supposed to be the most bioavailable, and species complexed to ligands. Although Zn2+ contents were high in media, Zn had no effect on the oxidoreductase production. Conversely, Cu highly induced the manganese peroxidase and laccase productions until 40 and 310 times when compared to unexposed controls. This inductive potential was highly correlated to Cu2+ contents in media. Furthermore, in poorly complexing media, the response threshold of oxidoreductases to Cu greatly decreased and an unexpected production of lignin peroxidase occurred.
Fichier principal
Vignette du fichier
11-BITE-def.pdf (235.19 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02648392 , version 1 (24-01-2023)

Identifiants

Citer

Jérémie D. Lebrun, Isabelle Lamy, Christian Mougin. Favouring the bioavailability of Zn and Cu to enhance the production of lignin-modifying enzymes in Trametes versicolor cultures. Bioresource Technology, 2011, 102 (3), pp.3103-3109. ⟨10.1016/j.biortech.2010.10.064⟩. ⟨hal-02648392⟩

Collections

INRA ECOSYS INRAE
20 Consultations
19 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More