Authenticated DNA from ancient wood remains
S. Liepelt
(1)
,
C. Sperisen
(2)
,
M. F. Deguilloux
(3)
,
R. J. Petit
(4)
,
R. Kissling
(5)
,
M. Spencer
(6)
,
J. L. Beaulieu
(7)
,
P. Taberlet
(8)
,
L. Gielly
(8)
,
B. Ziegenhagen
(1)
1
Faculty of Biology Nature Conservation Division
2 Snow and Landscape Research WSL
3 PACEA - De la Préhistoire à l'Actuel : Culture, Environnement et Anthropologie
4 BioGeCo - Biodiversité, Gènes et Ecosystèmes
5 Cytos Biotechnology AG
6 School of Biological Sciences
7 IMEP - Institut Méditerranéen d'Ecologie et de Paléoécologie
8 LECA - Laboratoire d'Ecologie Alpine
2 Snow and Landscape Research WSL
3 PACEA - De la Préhistoire à l'Actuel : Culture, Environnement et Anthropologie
4 BioGeCo - Biodiversité, Gènes et Ecosystèmes
5 Cytos Biotechnology AG
6 School of Biological Sciences
7 IMEP - Institut Méditerranéen d'Ecologie et de Paléoécologie
8 LECA - Laboratoire d'Ecologie Alpine
J. L. Beaulieu
- Fonction : Auteur
- PersonId : 173992
- IdHAL : jacques-louis-de-beaulieu
- IdRef : 061230472
Résumé
Background The reconstruction of biological processes and human activities during the last glacial cycle relies mainly on data from biological remains. Highly abundant tissues, such as wood, are candidates for a genetic analysis of past populations. While well-authenticated DNA has now been recovered from various fossil remains, the final 'proof' is still missing for wood, despite some promising studies. Scope The goal of this study was to determine if ancient wood can be analysed routinely in studies of archaeology and palaeogenetics. An experiment was designed which included blind testing, independent replicates, extensive contamination controls and rigorous statistical tests. Ten samples of ancient wood from major European forest tree genera were analysed with plastid DNA markers. Conclusions Authentic DNA was retrieved from wood samples up to 1000 years of age. A new tool for real-time vegetation history and archaeology is ready to use.
Format du dépôt | Notice |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Authenticated DNA from ancient wood remains
|
Résumé |
en
Background The reconstruction of biological processes and human activities during the last glacial cycle relies mainly on data from biological remains. Highly abundant tissues, such as wood, are candidates for a genetic analysis of past populations. While well-authenticated DNA has now been recovered from various fossil remains, the final 'proof' is still missing for wood, despite some promising studies. Scope The goal of this study was to determine if ancient wood can be analysed routinely in studies of archaeology and palaeogenetics. An experiment was designed which included blind testing, independent replicates, extensive contamination controls and rigorous statistical tests. Ten samples of ancient wood from major European forest tree genera were analysed with plastid DNA markers. Conclusions Authentic DNA was retrieved from wood samples up to 1000 years of age. A new tool for real-time vegetation history and archaeology is ready to use.
|
Auteur(s) |
S. Liepelt
1
, C. Sperisen
2
, M. F. Deguilloux
3
, R. J. Petit
4
, R. Kissling
5
, M. Spencer
6
, J. L. Beaulieu
7
, P. Taberlet
8
, L. Gielly
8
, B. Ziegenhagen
1
1
Faculty of Biology Nature Conservation Division
( 57939 )
- Karl-von-Frisch-Str. 2, D-35032 Marburg
- Allemagne
2
Snow and Landscape Research WSL
( 57940 )
- Zürcherstrasse 111, CH-8903 Birmensdorf
- Suisse
3
PACEA -
De la Préhistoire à l'Actuel : Culture, Environnement et Anthropologie
( 205557 )
- Université de Bordeaux - Bâtiment B8 - CS50023 - Allée Geoffroy-Saint-Hilaire - 33615 Pessac Cedex
- France
4
BioGeCo -
Biodiversité, Gènes et Ecosystèmes
( 52074 )
- 33612 Cestas
- France
5
Cytos Biotechnology AG
( 57941 )
- Wagistrasse 25 CH-8952 Schlieren
- Suisse
6
School of Biological Sciences
( 22416 )
- Royaume-Uni
7
IMEP -
Institut Méditerranéen d'Ecologie et de Paléoécologie
( 834 )
- Europole de l'Arbois BP 80 13545 Aix-en-Provence cedex 04 Faculté des Sciences de St Jérôme Av. Escadrille Normandie-Niemen - Boite 441 13397 Marseille cedex 20 Faculté des Sciences St Charles 3 place Victor Hugo 13331 Marseille cedex 03
- France
8
LECA -
Laboratoire d'Ecologie Alpine
( 723 )
- bat. D - Biologie 2233 Rue de la piscine - BP 53 38041 GRENOBLE CEDEX 9
- France
|
Comité de lecture |
Oui
|
Vulgarisation |
Non
|
Langue du document |
Anglais
|
Nom de la revue |
|
Audience |
Internationale
|
Date de publication |
2006
|
Volume |
98
|
Numéro |
5
|
Page/Identifiant |
1107-1111
|
Public visé |
Scientifique
|
Domaine(s) |
|
Indexation contrôlée |
|
Mots-clés |
en
fossil wood, Ancient DNA, trnL intron, probability of authenticity, Abies, Pinus, Fagus, Quercus
|
DOI | 10.1093/aob/mcl188 |
ProdINRA | 10989 |
PubMed Central | PMC3292245 |
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