This volume contains the proceedings of the 2nd International Conference on Innovative Intelligent Industrial Production and Logistics (IN4PL 2021), which was exceptionally held as a web-based event this year, due to the COVID-19 pandemic, from October 25-27, 2021. IN4PL is sponsored by the Institute for Systems and Technologies of Information, Control and Communication (INSTICC). This conference focuses on research and development involving innovative methods, software and hardware, whereby intelligent systems are applied to industrial production and logistics. This is currently related to the concept of industry 4.0 - an expression reflecting the trend towards automation and data exchange in manufacturing technologies and processes which include cyber-physical systems, the industrial internet of things, industrial robotics, cloud computing, cognitive computing and artificial intelligence. These technologies can be applied to industrial manufacturing and management as well as to supply-chain or logistic problems, involving for example transportation management or the optimization of operations. Different aspects were covered in three parallel tracks: Industry 4.0, Intelligent Manufacturing and Management, and Logistics and Operations Research. In addition to paper presentations, IN4PL’s program included two keynote talks delivered by internationally distinguished speakers: Joseph Sarkis (Worcester Polytechnic Institute, United States), Multi-stakeholder Digitalization: A Key to Building More Sustainable and Resilient Supply Chains?; and Fei Tao (Beihang University, China), Digital Twin: From Theory to Practice. The conference also included the IFAC/IFIP International Workshop on Enterprise Integration, Interoperability and Networking - EI2N 2021, chaired by Georg Weichhart, Milan Zdravkovic, Raquel Sanchis and Hervé Panetto. This workshop contributed to the community with oral presentations (full papers), which have also been included in the proceedings book. IN4PL received 26 paper submissions from 17 countries, of which 15% were accepted as full papers. The high quality of the papers received imposed difficult choices during the review process. To evaluate each submission, a double-blind paper review was performed by the Program Committee, whose members are highly qualified independent researchers in the IN4PL topic areas. All presented papers will be submitted for indexation by Scopus, Google Scholar, DBLP Computer Science Bibliography, Semantic Scholar, Microsoft Academic, EI (Elsevier Engineering Village Index), and Web of Science/Conference Proceedings Citation Index (Clarivate). A short list of papers presented at the conference will be selected for publication of extended and revised versions in the CCIS (Communications in Computer and Information Science) Series book by Springer. Another short list of papers will be invited for a post-conference special issue of the Springer Nature Computer Science Journal. All papers presented at this conference will be available at the SCITEPRESS Digital Library. We express our thanks to all participants. First to all the authors, whose quality work is the essence of this conference; secondly to all members of the Program Committee and auxiliary reviewers, who helped us with their expertise and valuable time. We also deeply thank the invited speakers for excellent contributions in sharing their knowledge and vision. Finally, we gratefully acknowledge the professional support of the INSTICC team for all organizational processes, especially given the need to introduce online streaming, forum management, direct messaging facilitation and other web-based activities in order to make it possible for IN4PL 2021 authors to present their work and share ideas with colleagues in spite of the logistic difficulties caused by the current pandemic situation. We wish you all an inspiring conference. We hope to meet you again next year for the 3rd edition of IN4PL, details of which will soon be available at https://in4pl.scitevents.org/.