Molecular characterization of genotype 2 and 4 hepatitis C virus isolates in French blood donors
Jean-François Cantaloube
(1)
,
Pierre Gallian
(1)
,
Syria Laperche
(2)
,
Marie-Hélène Elghouzzi
(3)
,
Yves Piquet
(3)
,
Françoise Bouchardeau
(3)
,
François Jordier
(1)
,
Philippe Biagini
(1, 4)
,
Houssam Attoui
(3)
,
Philippe de Micco
(1, 3)
Jean-François Cantaloube
- Fonction : Auteur
- PersonId : 764730
- ORCID : 0000-0001-6275-1668
- IdRef : 18162706X
Pierre Gallian
- Fonction : Auteur
- PersonId : 764731
- ORCID : 0000-0002-3310-5808
- IdRef : 067264506
Philippe Biagini
- Fonction : Auteur
- PersonId : 1182824
- IdHAL : philippe-biagini
- IdRef : 169831094
Houssam Attoui
- Fonction : Auteur
- PersonId : 744316
- IdHAL : houssam-attoui
- ORCID : 0000-0001-9639-9981
- IdRef : 169121119
Résumé
The subtype distribution of 142 genotype 2 and 97 genotype 4 hepatitis C virus (HCV) isolates from the sera of 1,319 volunteer blood donors in France was determined by gene sequencing and by phylogenetic analysis of the NS5B region and E1 envelope. Findings underlined a wide range of subtypes in both genotypes, that is, 20 in HCV-2 and 11 in HCV-4. Eighteen of these 31 subtypes had not been defined previously. Some subtypes, that is, 2a, 2b, 2c, 2i, 2k, 4a, and 4d, showed numerous strains while subtypes in donors from West Africa or Central Africa showed an endemic profile with only a few strains. A Bayesian coalescence approach was used to estimate the demographic history of each HCV subtype. The estimated mean dates of the most recent common ancestors (MRCA) were 1,889 (confidence interval (CI), 1,842-1,930) for HCV-2a, 1,886 (CI, 1,843-1,921) for HCV-2b, 1,791 (CI, 1,699-1,848) for HCV-2c, 1,846 (CI, 1,803-1,878) for HCV-2i, 1,911 (CI, 1,879-1,937) for HCV-4a, and 1,957 (CI, 1,943-1,967) for HCV-4d. The period of spread for subtype 2b, 2c, and 2i was between 1900 and 1960 whereas rapid exponential spread for subtype 2a, 4a, and 4d occurred in the 1960s. The inferred histories of population growth indicated that transmission rates differed according to HCV subtype. These results may help to predict the future burden of HCV in France.
Domaines
Anthropologie biologiqueFormat du dépôt | Notice |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Molecular characterization of genotype 2 and 4 hepatitis C virus isolates in French blood donors
|
Résumé |
en
The subtype distribution of 142 genotype 2 and 97 genotype 4 hepatitis C virus (HCV) isolates from the sera of 1,319 volunteer blood donors in France was determined by gene sequencing and by phylogenetic analysis of the NS5B region and E1 envelope. Findings underlined a wide range of subtypes in both genotypes, that is, 20 in HCV-2 and 11 in HCV-4. Eighteen of these 31 subtypes had not been defined previously. Some subtypes, that is, 2a, 2b, 2c, 2i, 2k, 4a, and 4d, showed numerous strains while subtypes in donors from West Africa or Central Africa showed an endemic profile with only a few strains. A Bayesian coalescence approach was used to estimate the demographic history of each HCV subtype. The estimated mean dates of the most recent common ancestors (MRCA) were 1,889 (confidence interval (CI), 1,842-1,930) for HCV-2a, 1,886 (CI, 1,843-1,921) for HCV-2b, 1,791 (CI, 1,699-1,848) for HCV-2c, 1,846 (CI, 1,803-1,878) for HCV-2i, 1,911 (CI, 1,879-1,937) for HCV-4a, and 1,957 (CI, 1,943-1,967) for HCV-4d. The period of spread for subtype 2b, 2c, and 2i was between 1900 and 1960 whereas rapid exponential spread for subtype 2a, 4a, and 4d occurred in the 1960s. The inferred histories of population growth indicated that transmission rates differed according to HCV subtype. These results may help to predict the future burden of HCV in France.
|
Auteur(s) |
Jean-François Cantaloube
1
, Pierre Gallian
1
, Syria Laperche
2
, Marie-Hélène Elghouzzi
3
, Yves Piquet
3
, Françoise Bouchardeau
3
, François Jordier
1
, Philippe Biagini
1, 4
, Houssam Attoui
3
, Philippe de Micco
1, 3
1
UAABC -
Anthropologie Bio-Culturelle
( 942 )
- Faculté de Médecine - Secteur Nord Batiment A - CS80011 Bd Pierre Dramard 13344 MARSEILLE Cedex 15 FRANCE
- France
2
Centre National de Référence Virus des hépatites B, C et Delta
( 89367 )
- France
3
EFS - Alpes-Méditerranée -
Etablissement Français du Sang - Alpes-Méditerranée
( 121596 )
- 149 bd Baille 13385, Marseille
- France
4
ADES -
Anthropologie bio-culturelle, Droit, Ethique et Santé
( 186857 )
- Faculté de Médecine Secteur Nord 51, Boulevard Pierre Dramard 13344 MARSEILLE CEDEX 15
- France
|
Comité de lecture |
Oui
|
Vulgarisation |
Non
|
Langue du document |
Anglais
|
Nom de la revue |
|
Audience |
Non spécifiée
|
Date de publication |
2008
|
Volume |
80
|
Page/Identifiant |
1732-1739
|
Domaine(s) |
|
Mots-clés |
en
HCV, genotyping, phylogeny, coalescence
|
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