High performance liquid chromatography of slightly soluble brominated indigoids from Tyrian purple - HAL Accéder directement au contenu
Article dans une revue Journal of Chromatography A Année : 2011

High performance liquid chromatography of slightly soluble brominated indigoids from Tyrian purple

Résumé

Attempts at identification of mollusc species producing Tyrian purple from archaeological material are usually done with high performance liquid chromatography in the reversed-phase system, but the peaks obtained are often wide and asymmetric. This is due to the low solubility of the indigoids and their brominated derivatives in the mobile phase, especially 6,6 -dibromoindigotin, which is soluble in only few, particular solvents. Our study focused on improving both symmetry and peak height for more precise quantification. The influence of various factors was evaluated: stationary phase characteristics, mobile phase composition, elution gradient parameters and temperature on the peak shape of the main components of Tyrian purple. The best results were obtained using highly retentive, but moderately bonded ODS stationary phases (about 2.8 .mol m −2 ), percolated with gradient of acetonitrile with acidified aqueous mobile phases (0.1% strong acid) at elevated temperatures (70 ◦ C). The upper quantification limit for 6,6 -dibromoindigotin was improved by over 350%, between standard and optimised systems. Using them, the detection and quantification of trace Tyrian purple components (less than 0.15%) aside from major indigoids becomes possible. Consequently, for the first time, the new analogues of brominated and unbrominated indirubins were found in the shellfish purple from Hexaplex trunculus.
Présentation d'un système analytique optimisé pour l'identification de la pourpre de Muricidés dans les textiles archéologiques.
Loading...
Fichier non déposé

Dates et versions

halshs-00974059, version 1 (05-04-2014)

Identifiants

  • HAL Id : halshs-00974059 , version 1

Citer

Witold Nowik, Regina Marcinowska, Katia Kusyk, Dominique Cardon, Marek Trojanowicz. High performance liquid chromatography of slightly soluble brominated indigoids from Tyrian purple. Journal of Chromatography A, 2011, 1218, pp.1244-1252. ⟨halshs-00974059⟩
226 Consultations
0 Téléchargements
Dernière date de mise à jour le 20/04/2024
comment ces indicateurs sont-ils produits

Partager

Gmail Facebook Twitter LinkedIn Plus