Amy2B copy number variation reveals starch diet adaptation in ancient European dogs
Morgane Ollivier
(1, 2)
,
Anne Tresset
(3)
,
Fabiola Bastian
(2, 1)
,
Laëtitia Lagoutte
(4)
,
Erik Axelsson
(5)
,
Maja-Louise Arendt
(5)
,
Adrian Balasescu
(6)
,
Mikhail V. Sablin
(7)
,
Laure Salanova
(8)
,
Catherine Haenni
(2)
,
Jean-Denis Vigne
(3)
,
Christophe Hitte
(4)
,
Marjan Mashkour
(3)
1
LECA -
Laboratoire d'Ecologie Alpine
2 IGFL - Institut de Génomique Fonctionnelle de Lyon
3 AASPE - Archéozoologie, archéobotanique : sociétés, pratiques et environnements
4 IGDR - Institut de Génétique et Développement de Rennes
5 Science for Life Laboratory
6 MNIR - Musée National d'Histoire de Bucarest
7 Zoological Institute of Russian Academy of Sciences
8 AOROC - Archéologie et Philologie d'Orient et d'Occident
2 IGFL - Institut de Génomique Fonctionnelle de Lyon
3 AASPE - Archéozoologie, archéobotanique : sociétés, pratiques et environnements
4 IGDR - Institut de Génétique et Développement de Rennes
5 Science for Life Laboratory
6 MNIR - Musée National d'Histoire de Bucarest
7 Zoological Institute of Russian Academy of Sciences
8 AOROC - Archéologie et Philologie d'Orient et d'Occident
Morgane Ollivier
- Fonction : Auteur
- PersonId : 746231
- IdHAL : morgane-ollivier
- ORCID : 0000-0002-8361-4221
- IdRef : 148722865
Anne Tresset
- Fonction : Auteur
- PersonId : 899806
Fabiola Bastian
- Fonction : Auteur
- PersonId : 170363
- IdHAL : fabiola-bastian
- IdRef : 253130239
Laëtitia Lagoutte
- Fonction : Auteur
- PersonId : 744844
- IdHAL : laetitia-lagoutte
Laure Salanova
- Fonction : Auteur
- PersonId : 8487
- IdHAL : laure-salanova
- ORCID : 0000-0002-1365-2716
- IdRef : 055117368
Catherine Haenni
- Fonction : Auteur
- PersonId : 6960
- IdHAL : catherine-hanni
- IdRef : 080107273
Jean-Denis Vigne
- Fonction : Auteur
- PersonId : 174348
- IdHAL : jean-denis-vigne
- ORCID : 0000-0002-7367-7552
- IdRef : 029288126
Christophe Hitte
- Fonction : Auteur
- PersonId : 1202813
Marjan Mashkour
- Fonction : Auteur
- PersonId : 176173
- IdHAL : marjan-mashkour
- ORCID : 0000-0003-3630-9459
- IdRef : 057725977
Résumé
Extant dog and wolf DNA indicates that dog domestication was accompanied by the selection of a series of duplications on the Amy2B gene coding for pancreatic amylase. In this study, we used a palaeogenetic approach to investigate the timing and expansion of the Amy2B gene in the ancient dog populations of Western and Eastern Europe and Southwest Asia. Quantitative polymerase chain reaction was used to estimate the copy numbers of this gene for 13 ancient dog samples, dated to between 15 000 and 4000 years before present (cal. BP). This evidenced an increase of Amy2B copies in ancient dogs from as early as the 7th millennium cal. BP in Southeastern Europe. We found that the gene expansion was not fixed across all dogs within this early farming context, with ancient dogs bearing between 2 and 20 diploid copies of the gene. The results also suggested that selection for the increased Amy2B copy number started 7000 years cal. BP, at the latest. This expansion reflects a local adaptation that allowed dogs to thrive on a starch rich diet, especially within early farming societies, and suggests a biocultural coevolution of dog genes and human culture.
Format du dépôt | Notice |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
Amy2B copy number variation reveals starch diet adaptation in ancient European dogs
|
Résumé |
en
Extant dog and wolf DNA indicates that dog domestication was accompanied by the selection of a series of duplications on the Amy2B gene coding for pancreatic amylase. In this study, we used a palaeogenetic approach to investigate the timing and expansion of the Amy2B gene in the ancient dog populations of Western and Eastern Europe and Southwest Asia. Quantitative polymerase chain reaction was used to estimate the copy numbers of this gene for 13 ancient dog samples, dated to between 15 000 and 4000 years before present (cal. BP). This evidenced an increase of Amy2B copies in ancient dogs from as early as the 7th millennium cal. BP in Southeastern Europe. We found that the gene expansion was not fixed across all dogs within this early farming context, with ancient dogs bearing between 2 and 20 diploid copies of the gene. The results also suggested that selection for the increased Amy2B copy number started 7000 years cal. BP, at the latest. This expansion reflects a local adaptation that allowed dogs to thrive on a starch rich diet, especially within early farming societies, and suggests a biocultural coevolution of dog genes and human culture.
|
Auteur(s) |
Morgane Ollivier
1, 2
, Anne Tresset
3
, Fabiola Bastian
2, 1
, Laëtitia Lagoutte
4
, Erik Axelsson
5
, Maja-Louise Arendt
5
, Adrian Balasescu
6
, Mikhail V. Sablin
7
, Laure Salanova
8
, Catherine Haenni
2
, Jean-Denis Vigne
3
, Christophe Hitte
4
, Marjan Mashkour
3
1
LECA -
Laboratoire d'Ecologie Alpine
( 1041894 )
- bat. D - Biologie 2233 Rue de la piscine - BP 53 38041 GRENOBLE CEDEX 9
- France
2
IGFL -
Institut de Génomique Fonctionnelle de Lyon
( 39280 )
- Ecole Normale Supérieure de Lyon 46 allée d'Italie 69364 Lyon CEDEX07
- France
3
AASPE -
Archéozoologie, archéobotanique : sociétés, pratiques et environnements
( 118105 )
- Muséum national d'Histoire naturelle - Case postale 56 - 55 rue Buffon - 75005 Paris
- France
4
IGDR -
Institut de Génétique et Développement de Rennes
( 187162 )
- Faculté de Médecine - CS 34317 2 Av du Professeur Léon Bernard 35043 Rennes Cedex
- France
5
Science for Life Laboratory
( 161201 )
- Box 582, SE-751 23, Uppsala
- Suède
6
MNIR -
Musée National d'Histoire de Bucarest
( 238412 )
- Calea Victoriei, nr.12, sect. 3, cod poştal 030026, Bucureşti,
- Roumanie
7
Zoological Institute of Russian Academy of Sciences
( 11724 )
- Universiteskaya emb. 1 119034 St Petersburg
- Russie
8
AOROC -
Archéologie et Philologie d'Orient et d'Occident
( 226098 )
- CNRS : UMR8546 - Ecole Normale Supérieure de Paris - ENS Paris - 45 Rue d'Ulm 75230 PARIS CEDEX 05
- France
|
Page/Identifiant |
160449
|
Numéro |
11
|
Volume |
3
|
Langue du document |
Anglais
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication |
2016-11-09
|
Nom de la revue |
|
Date de publication électronique |
2016-11-09
|
Collaboration/Projet |
|
Domaine(s) |
|
Financement |
|
Mots-clés |
en
Dog, Genetics, Agrarian history
|
DOI | 10.1098/rsos.160449 |
Pubmed Id | 28018628 |
PubMed Central | PMC5180126 |
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