Dynamic full-field optical coherence tomography as complementary tool in fungal diagnostics
Thomas Maldiney
(1, 2)
,
Jean-Marie Chassot
(3)
,
Claude Boccara
(3)
,
Mathieu Blot
(2, 4)
,
Lionel Piroth
(4, 5)
,
Pierre-Emmanuel Charles
(2, 4)
,
Dea Garcia-Hermoso
(6, 7)
,
Fanny Lanternier
(6, 7, 8)
,
Frédéric Dalle
(4, 9)
,
Marc Sautour
(4, 9)
1
Centre Hospitalier Chalon-sur-Saône William Morey
2 LIPNESS - Equipe LIPNESS (LNC - U1231)
3 IL - Institut Langevin - Ondes et Images (UMR7587)
4 CHU Dijon
5 CIC-EC - Centre d'Investigation Clinique 1432 (Dijon) - Epidemiologie Clinique/Essais Cliniques
6 Mycologie moléculaire - Molecular Mycology
7 CNRMA - Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals
8 Hôpital Necker - Enfants Malades [AP-HP]
9 PAM - Procédés Alimentaires et Microbiologiques [Dijon]
2 LIPNESS - Equipe LIPNESS (LNC - U1231)
3 IL - Institut Langevin - Ondes et Images (UMR7587)
4 CHU Dijon
5 CIC-EC - Centre d'Investigation Clinique 1432 (Dijon) - Epidemiologie Clinique/Essais Cliniques
6 Mycologie moléculaire - Molecular Mycology
7 CNRMA - Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals
8 Hôpital Necker - Enfants Malades [AP-HP]
9 PAM - Procédés Alimentaires et Microbiologiques [Dijon]
Thomas Maldiney
- Fonction : Auteur
- PersonId : 1273538
- ORCID : 0000-0002-7514-2113
Dea Garcia-Hermoso
- Fonction : Auteur
- PersonId : 1273549
- IdHAL : dea-garcia-hermoso
- ORCID : 0000-0001-5288-2904
Résumé
Histopathology and microscopic examination of infected tissue are the gold standards to prove the diagnosis of invasive fungal infection (IFI). Yet, they suffer from essential limitations that hamper rapid diagnosis and require the future development of new imaging tools dedicated to fungal diagnostics. To this end, the present work introduces the first use of dynamic full-field optical coherence tomography (D-FF-OCT) for the visualization of microscopic filamentous fungi. Data collected from the observation of three different fungal species (Nannizzia gypsea, Aspergillus fumigatus and Rhizopus arrhizus) confirm the ability of D-FF-OCT to visualize not only the main structures of all selected fungal species (hyphae, spores, conidia, sporulating structures), but also the metabolic activity of the organisms, which could provide additional help in the future to better characterize the signature of each fungal structure. These results demonstrate how D-FF-OCT could serve as potential complementary tool for rapid diagnosis of IFI in both intensive and non-intensive care units.
Domaines
Sciences de l'Homme et SociétéFormat du dépôt | Notice |
---|---|
Type de dépôt | Article dans une revue |
Résumé |
en
Histopathology and microscopic examination of infected tissue are the gold standards to prove the diagnosis of invasive fungal infection (IFI). Yet, they suffer from essential limitations that hamper rapid diagnosis and require the future development of new imaging tools dedicated to fungal diagnostics. To this end, the present work introduces the first use of dynamic full-field optical coherence tomography (D-FF-OCT) for the visualization of microscopic filamentous fungi. Data collected from the observation of three different fungal species (Nannizzia gypsea, Aspergillus fumigatus and Rhizopus arrhizus) confirm the ability of D-FF-OCT to visualize not only the main structures of all selected fungal species (hyphae, spores, conidia, sporulating structures), but also the metabolic activity of the organisms, which could provide additional help in the future to better characterize the signature of each fungal structure. These results demonstrate how D-FF-OCT could serve as potential complementary tool for rapid diagnosis of IFI in both intensive and non-intensive care units.
|
Titre |
en
Dynamic full-field optical coherence tomography as complementary tool in fungal diagnostics
|
Auteur(s) |
Thomas Maldiney
1, 2
, Jean-Marie Chassot
3
, Claude Boccara
3
, Mathieu Blot
2, 4
, Lionel Piroth
4, 5
, Pierre-Emmanuel Charles
2, 4
, Dea Garcia-Hermoso
6, 7
, Fanny Lanternier
6, 7, 8
, Frédéric Dalle
4, 9
, Marc Sautour
4, 9
1
Centre Hospitalier Chalon-sur-Saône William Morey
( 408481 )
- 4, rue Capitaine Drillien, 71321 Chalon-sur-Saône
- France
2
LIPNESS -
Equipe LIPNESS (LNC - U1231)
( 1096467 )
- Université de Bourgogne - Faculté de médecine - 7, Bld Jeanne d'Arc - BP 87900 - 21079 DIJON CEDEX
- France
3
IL -
Institut Langevin - Ondes et Images (UMR7587)
( 1004908 )
- ESPCI, 1 Rue Jussieu 75238 Paris Cedex 05
- France
4
CHU Dijon
( 35051 )
- BP1542, 21034 Dijon cedex
- France
5
CIC-EC -
Centre d'Investigation Clinique 1432 (Dijon) - Epidemiologie Clinique/Essais Cliniques
( 2625 )
- Faculté de Médecine - - BP 87900 - 7 Bd Jeanne d'Arc - 21079 DIJON CEDEX
- France
6
Mycologie moléculaire - Molecular Mycology
( 1090784 )
- Département de mycologie
25-28, rue du Docteur Roux
75724 Paris cedex 15
- France
7
CNRMA -
Centre National de Référence Mycoses Invasives et Antifongiques - National Reference Center Invasive Mycoses & Antifungals
( 1090832 )
- Département de Mycologie
Unité de Mycologie Moléculaire
25-28 rue du Docteur Roux
75724 Paris cedex 15
- France
8
Hôpital Necker - Enfants Malades [AP-HP]
( 414766 )
- 149 Rue de Sèvres 75015 Paris
- France
9
PAM -
Procédés Alimentaires et Microbiologiques [Dijon]
( 1096484 )
- Université Bourgogne Franche-Comté - Institut Agro Dijon - Bâtiment Epicure - 1 esplanade Erasme - F-21000 Dijon
- France
|
Vulgarisation |
Non
|
Audience |
Internationale
|
Comité de lecture |
Oui
|
Langue du document |
Anglais
|
Nom de la revue |
|
Date de publication |
2022-11
|
Volume |
32
|
Numéro |
4
|
Page/Identifiant |
101303
|
Financement |
|
Domaine(s) |
|
Mots-clés |
en
Full-field optical coherence tomography, Fungal diagnostics, Invasive fungal infections, Dynamic contrast, Fungal metabolism
|
DOI | 10.1016/j.mycmed.2022.101303 |
Pubmed Id | 35732095 |
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