This paper seeks to shed new light on the latest discovered occupation period at Ulug-depe: the Hellenistic and Parthian occupation. Ulug-depe displays the longest continuous stratigraphic sequence of southern Central Asia, from the Late Neolithic to the Middle Iron Age. After its abandonment at the end of the Middle Iron Age period, the site was reoccupied during the late 1st millennium BC. Although the archaeological levels relating to this period are badly preserved, extended research led by the joint Turkmen-French Archaeological Expedition (MAFTur) has succeeded in unearthing this occupation in many places of the site and has studied a varied pottery assemblage. Its study suggests Ulug-depe was located strategically in the central part of the Kopetdag during the last centuries of the 1st millennium BCE.